Foton Sayımı ve Zamanlaması
TCSPC and Time Tagging Electronics
PicoQuant offers several modules for Time-Correlated Single Photon Counting (TCSPC), Multi-Channel Scaling (MCS), coincidence correlations, or event timing with temporal resolutions between picoseconds and milliseconds. Single channel as well as multichannel versions with either USB or PCIe interface can be provided. All units work in the histogramming mode and offer two time tagging modes. Their high quality and reliability are expressed by a unique 5-year limited warranty. The following table lists the main differences: | |
HydraHarp 400
TimeHarp 260
TCSPC and MCS board with PCIe interface
- • One or two independent input channels and common synch channel (up to 100 MHz)
- • Two models with either 25 ps (PICO model) or 250 ps (NANO model) base resolution
- • “Long range mode” option for PICO model with 2.5 ns base resolution
- • Ultra short dead time (< 25 ns for PICO model, < 2 ns for NANO model)
Photon Counting Detectors
The available photon counting detectors include photomultiplier tubes (PMT), Hybrid photomultiplier detectors (HPD) and Single Photon Avalanche Diodes (SPAD) that cover different spectral ranges between the UV and the NIR. All detectors feature timing resolutions in the picosecond range, depending on model and operating conditions. The following table gives a rough overview about the key parameters:
Software
PicoQuant offers specialized software for the analysis of fluorescence measurements. This includes a software for global fluorescence decay data analysis as well as a specialized imaging and correlation software, which supports methods like FLIM and FLIM-FRET, FCS, FCCS, FLCS, anisotropy and the analysis of fluorescence time traces. | |
SymPhoTime 64
Fluorescence Lifetime Imaging and Correlation Software
- • Point, 2D, and 3D TTTR data acquisition with online preview of FLIM, FCS, time-traces, or TCSPC histograms
- • FLIM, fast FLIM, FLIM-FRET
- • FCS, FCCS, FLCS, PIE-FCS, coincidence correlation, total correlation
- • FRET, PIE-FRET, fluorescence time traces, anisotropy
FluoFit
Global Fluorescence Decay Data Analysis Software
- • Tail fitting or iterative reconvolution, maximum likelihood estimation
- • Exponential decay models up to 4th order
- • Lifetime distribution models (Gaussian, Lorentzian, Stretched Exponentials)
- • Anisotropy analysis with an exponential decay model up to 4th order
FluoFit
Global Fluorescence Decay Data Analysis Software
- • Tail fitting or iterative reconvolution, maximum likelihood estimation
- • Exponential decay models up to 4th order
- • Lifetime distribution models (Gaussian, Lorentzian, Stretched Exponentials)
- • Anisotropy analysis with an exponential decay model up to 4th order
Accessories
Accessories for photon counting experiments include a router for the PicoHarp 300 TCSPC module, a specialized dual channel power supply for SPADs, a signal delayer with picosecond resolution and a trigger diode. Signal conditioners (inverter, attenuator, pre-amplifier or splitter) as well as signal cables can also be provided. | |
PHR 800
Universal 4-channel detector router for the PicoHarp 300
- • Allows simultaneous measurement of 4 detector signals
- • Adjustable input polarity and trigger threshold
- • Predefined settings for TTL or NIM signals provided by SPAD detectors
- • Optional Constant Fraction Discriminators for PMTs, Hybrid-PMTs and MCP-PMTs